tcyA [2018-05-04 12:44:57]

cystine ABC transporter (binding protein)

locus: BSU03610

pI: 9.12

mw: 29.32 kDa

protein length: 268 aa

gene length: 804 bp

function: cystine uptake

product: cystine ABC transporter (binding protein)

essential: no

synonyms: yckK

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.2|Uptake of amino acids]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.9|Biosynthesis/ acquisition of cysteine]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 410,656 → 411,462

The protein

Protein family

• bacterial solute-binding protein 3 family (according to Swiss-Prot)

[SW|Localization]

• attached to the membrane via [protein|9400DF25487461E3738DDDC72BDDB1589281409D|TcyB] [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|2056F8EEDF9D3027CFD808E8D0C5DF544DBDD3F3|tcyA]-[gene|9400DF25487461E3738DDDC72BDDB1589281409D|tcyB]-[gene|B453986691E1231A1C565D469A2A60EBFF2F6BD3|tcyC]
  description: [Pubmed|22383849,15262924]

  regulation

  • strongly repressed in response to glucose starvation in M9 medium [Pubmed|23033921]

Biological materials

Mutant

• BKE03610 (Δ[gene|2056F8EEDF9D3027CFD808E8D0C5DF544DBDD3F3|tcyA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTATTCCCCACTTTTC, downstream forward: _UP4_GAAGATGTTTCTAAATAATC
• BKK03610 (Δ[gene|2056F8EEDF9D3027CFD808E8D0C5DF544DBDD3F3|tcyA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTATTCCCCACTTTTC, downstream forward: _UP4_GAAGATGTTTCTAAATAATC

References

• 15262924,10092453,7551042,23033921